Cell Growth

Measurement of living cells using the Countess II Automated Cell Counter, a benchtop automated cell counting system.

This instrument enables accurate counting of:

• Live cells
• Dead cells
• Total cell number

The test is performed using trypan blue staining, a widely used method for distinguishing viable and non-viable cells.

The measurement of how rapidly cells increase in number over time. In general, cells demonstrate an exponential growth pattern during the proliferation phase.
Our laboratory monitors cell growth daily over a continuous 5-day period. Results are reported as:

• Daily cell counts
• Growth trend graphs

This provides a clear overview of cellular proliferation performance under defined culture conditions.

Population Doubling Time (PDT) is the time required for a cell population to double from its initial number under specified culture conditions.
PDT is an important indicator used to evaluate the growth capacity of cells:

• A shorter PDT indicates faster cell proliferation
• A longer PDT may indicate slower growth or suboptimal culture conditions

This parameter is commonly used to assess cell quality, culture efficiency, and overall cellular performance.